Rapid communication: polymorphism in a bovine heart-fatty acid binding protein-like (H-FABPL) DNA sequence.

نویسندگان

  • P A Tank
  • D Pomp
چکیده

Polymorphism. Two alleles of a DNA region (HFABPL) with high homology to the bovine heart-fatty acid binding protein (H-FABP) cDNA were detected through RFLP analysis of PCR products digested with the restriction enzyme RsaI. Primer Source. Primers were designed based on the bovine cDNA sequence for H-FABP (Billich et al., 1988) to amplify between nucleotides 66-676. The primer s quences were as follows: 5' primer, 5'-TACCTGGAAGTTAGTGGACAGC-3' and 3' primer:

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Rapid Diagnosis of acute kidney injury (AKI) associated with cardiac surgery, using the liver type fatty acid binding protein (L-FABP) biomarker

Abstract Background and objectives: cardiac surgery is often associated with acute kidney injury (AKI). Nowadays, AKI is typically diagnosed by an increase in serum creatinine, which is a delayed and unreliable biomarker. Recent studies recommended using the liver type fatty acid binding protein (L-FABP) as an early biomarker. Material and Methods: The urine samples of 18 adult patients undergo...

متن کامل

Rapid purification of HU protein from Halobacillus karajensis

The histone-like protein HU is the most-abundant DNA-binding protein in bacteria. The HU protein non-specifically binds and bends DNA as a hetero- or homodimer, and can participate in DNA supercoiling and DNA condensation. It also takes part in DNA functions such as replication, recombination, and repair. HU does not recognize any specific sequences but shows a certain degree of specificity to ...

متن کامل

Detection of New Silent Mutation at 348 bp Position in a CD18 Gene in Holstein Cattle Normal and Heterozygous for Bovine Leukocyte Adhesion Deficiency Syndrome

In India, Holstein and its crosses are being used extensively in breeding programmes and all these breeding bulls are screened for autosomal recessive genes. Blood samples are collected in ethylenediaminetetraacetic acid (EDTA) coated tubes and DNA was isolated by using phenol-chloroform method. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) wereperformed by using...

متن کامل

Differential expression of adipose- and heart-type fatty acid binding proteins in hibernating ground squirrels.

The up-regulation of heart- and adipose-type fatty acid binding proteins (H-FABPs and A-FABPs) was detected during hibernation in brown adipose tissue (BAT) of 13-lined ground squirrels, Spermophilus tridecemlineatus, using a commercial rat cDNA array. Full length cDNAs encoding H-FABPs and A-FABPs were subsequently retrieved from a BAT cDNA library. These cDNAs were used to probe Northern blot...

متن کامل

Effect of Saffron Extract and Aerobic Exercises on Troponin T and Heart-Type Fatty Acid Binding Protein in Type 2 Diabetes Patients

Objective: Exercise and nutrition as life style modification strategies for the prevention and control of diabetes are considered. The aim of present study was to assess the effects of saffron consumption and aerobic exercises on serum levels of Heart-type fatty acid binding protein (HFABP) and Troponin T in type2 diabetes (T2DM) patients. Materials and Methods: This study was a quasi-experime...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Journal of animal science

دوره 73 3  شماره 

صفحات  -

تاریخ انتشار 1995